Recombinant CMKLR1 antibody (AA 8-32)

Catalog No. ABIN7072487

Product Details anti-CMKLR1 Antibody

Target: CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 8-32

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CMKLR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Purpose: Anti-CMKLR1 [BZ194], Rabbit IgG, kappa

Specificity: This antibody is specific for murine serpentine receptor mCMKLR1 (aka ChemR23, DEZ). mCMKLR1 is a novel protein possessing high homology with members of the chemoattractant receptor family, and binds the chemoattractant chemerin. Selective mCMKLR1 expression has been demonstrated by mouse macrophages ex vivo, and by dendritic cell precursors cultured in vitro.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Wistar Furth rats with the mCMKLR1 peptide/KLH conjugate. The immunizing amino-terminal mCMKLR1 peptide with the sequence NH2-DSGIYDDEYSDGFGYFVDLEEASPWC-COOH, corresponding to residues residues 8-32 of mCMKLR1, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

Clone: BZ194

Isotype: IgG kappa

Application Details

Application Notes: The anti-mCMKLR1 BZ194 generates a robust staining signal when used in flow cytometry applications with mCMKLR1+ cells (Zabel et al., 2006). This antibody, together with other mAbs directed against chemokine receptors, could be used to determine the leukocyte expression profile of receptors during homeostasis or inflammation, to elucidate the role of various receptors in coordinating the immune response and in leukocyte development, and to identify other proteins interacting with the chemokine receptor. In particular, this antibody has been used to evaluate the functional responses of mCMKLR1-expressing cells to chemerin (Zabel et al., 2006), to demonstrate the esssential role of natural killer cells in resolution of antigen-induced inflammation in mice (Anuforo et al., 2018), to indicate a potential role for chemerin and CMKLR1 in the regulation of inflammatory responses in the tumor microenvironment (Rama et al, 2011), to visualise immediate immune responses to pioneer metastatic cells in the lung (Headley et al., 2016), and to investigate the regulatory role of chemerin in lung tumorigenesis and cancer-associated inflammation (Unver et al, 2018).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CMKLR1

Target: CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))

Alternative Name: CMKLR1 (CMKLR1 Products)

Background: Chemerin receptor, CHEMERINR, chemokine receptor-like 1, Chemokine-like receptor 1, ChemR23, CMKLR1, DEZ, G-protein coupled receptor ChemR23, G-protein coupled receptor DEZ, orphan G-protein coupled receptor, Dez, Resolvin E1 receptor, RVER1, Gpcr27, MGC126105, MGC126106

UniProt: P97468