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Recombinant CMKLR1 antibody (AA 8-32)

CMKLR1 Reactivity: Mouse WB, ELISA, IHC, IF, FACS, IP, Func Host: Rabbit Chimeric BZ194 unconjugated Recombinant Antibody
Catalog No. ABIN7072487
  • Target See all CMKLR1 Antibodies
    CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))
    Antibody Type
    Recombinant Antibody
    Binding Specificity
    • 16
    • 15
    • 9
    • 7
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 8-32
    Reactivity
    • 70
    • 41
    • 24
    • 18
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 71
    • 3
    • 2
    Rabbit
    Clonality
    • 70
    • 6
    Chimeric
    Conjugate
    • 31
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CMKLR1 antibody is un-conjugated
    Application
    • 56
    • 26
    • 26
    • 22
    • 19
    • 13
    • 8
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)
    Purpose
    Anti-CMKLR1 [BZ194], Rabbit IgG, kappa
    Specificity
    This antibody is specific for murine serpentine receptor mCMKLR1 (aka ChemR23, DEZ). mCMKLR1 is a novel protein possessing high homology with members of the chemoattractant receptor family, and binds the chemoattractant chemerin. Selective mCMKLR1 expression has been demonstrated by mouse macrophages ex vivo, and by dendritic cell precursors cultured in vitro.
    Characteristics

    Original Species of Ab: Rat

    Original Format of Ab: IgG2a

    Purification
    Protein A affinity purified
    Immunogen
    This antibody was raised by immunising Wistar Furth rats with the mCMKLR1 peptide/KLH conjugate. The immunizing amino-terminal mCMKLR1 peptide with the sequence NH2-DSGIYDDEYSDGFGYFVDLEEASPWC-COOH, corresponding to residues residues 8-32 of mCMKLR1, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
    Clone
    BZ194
    Isotype
    IgG kappa
    Top Product
    Discover our top product CMKLR1 Primary Antibody
  • Application Notes
    The anti-mCMKLR1 BZ194 generates a robust staining signal when used in flow cytometry applications with mCMKLR1+ cells (Zabel et al., 2006). This antibody, together with other mAbs directed against chemokine receptors, could be used to determine the leukocyte expression profile of receptors during homeostasis or inflammation, to elucidate the role of various receptors in coordinating the immune response and in leukocyte development, and to identify other proteins interacting with the chemokine receptor. In particular, this antibody has been used to evaluate the functional responses of mCMKLR1-expressing cells to chemerin (Zabel et al., 2006), to demonstrate the esssential role of natural killer cells in resolution of antigen-induced inflammation in mice (Anuforo et al., 2018), to indicate a potential role for chemerin and CMKLR1 in the regulation of inflammatory responses in the tumor microenvironment (Rama et al, 2011), to visualise immediate immune responses to pioneer metastatic cells in the lung (Headley et al., 2016), and to investigate the regulatory role of chemerin in lung tumorigenesis and cancer-associated inflammation (Unver et al, 2018).
    Comment

    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))
    Alternative Name
    CMKLR1 (CMKLR1 Products)
    Background
    Chemerin receptor, CHEMERINR, chemokine receptor-like 1, Chemokine-like receptor 1, ChemR23, CMKLR1, DEZ, G-protein coupled receptor ChemR23, G-protein coupled receptor DEZ, orphan G-protein coupled receptor, Dez, Resolvin E1 receptor, RVER1, Gpcr27, MGC126105, MGC126106
    UniProt
    P97468
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