Recombinant CD276 antibody (Extracellular Domain)

Catalog No. ABIN7072434

Product Details anti-CD276 Antibody

Target: CD276

Antibody Type: Recombinant Antibody

Binding Specificity: AA 1-242, Extracellular Domain

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD276 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Purpose: Anti-B7-H3 [MJ18], Rabbit IgG, kappa

Specificity: This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.

Clone: MJ18

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used to detect murine B7-H3 by flow cytometry. It blocks B7-H3 signalling and can supress priming of Th2-responses and thereby the development of asthmatic responses in an ovalbumin-induced asthma model in BALB/c mice.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD276

Target: CD276

Alternative Name: B7-H3 (CD276 Products)

Background: CD276, B7H3

UniProt: Q8VE98

Pathways: Cancer Immune Checkpoints