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Recombinant MHC, Class I antibody

Reactivity: Mouse IHC, IP, IA Host: Rabbit Chimeric R1-21-2 unconjugated Recombinant Antibody
Catalog No. ABIN7072426
  • Target See all MHC, Class I Antibodies
    MHC, Class I
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 71
    • 36
    • 13
    • 9
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 1
    Mouse
    Host
    • 57
    • 46
    • 5
    • 1
    Rabbit
    Clonality
    • 71
    • 36
    • 2
    Chimeric
    Conjugate
    • 35
    • 14
    • 12
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MHC, Class I antibody is un-conjugated
    Application
    • 68
    • 51
    • 29
    • 22
    • 20
    • 14
    • 13
    • 13
    • 12
    • 11
    • 7
    • 5
    • 4
    • 1
    Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunoassay (IA)
    Purpose
    Anti-MHC I [R1-21.2], Rabbit IgG, kappa
    Specificity
    This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.
    Characteristics

    Original Species of Ab: Rat

    Original Format of Ab: IgG2b

    Purification
    Protein A affinity purified
    Immunogen
    This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
    Clone
    R1-21-2
    Isotype
    IgG kappa
    Top Product
    Discover our top product MHC, Class I Primary Antibody
  • Application Notes
    This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. It has been employed to assess MHC class I expression in frozen, acetone-fixed mouse brain sections by immunohistochemistry (Miralvès et al, 2007), and to stain paraffin-embedded sections of mouse liver for H-2Kb expression (Limmer et al, 2005). It has also been used to precipitate mouse H-2Kk and H-2Dk class I molecules from detergent lysates of Ltk3.3/1 cells (Rada et al, 1990), and to stain H-2Kb and H-2Kk-expressing cells in FACS analysis (Bendelac, 1995). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983).
    Comment

    This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    MHC, Class I
    Alternative Name
    MHC I (MHC, Class I Products)
    Background
    R1.21.2, H-2, MHC-I, MHC Class I
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