Recombinant MHC, Class I antibody

Catalog No. ABIN7072424

Product Details anti-MHC, Class I Antibody

Target: MHC, Class I

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This MHC, Class I antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunofluorescence (IF), Immunoassay (IA)

Purpose: Anti-MHC I [R1-9.6], Rabbit IgG, kappa

Specificity: This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody recognises a determinant distinct from those detected by most common mouse monoclonal anti-H-2 antibodies.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.

Clone: R1-9-6

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds murine H-2 MHC class I molecules and has been used in both structural and biochemical studies. This antibody has been used in immunoprecipitation analysis (Robinson, 1987) and in immunofluorescence assays for MHC-I surface expression (Karacsonyi et al., 2004) and MHC-I endocytosis (Knorr et al., 2009). This antibody has also been employed to induce clustering of cell surface MHC-I in co-patching assays (Knorr et al., 2009). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells and assess its strain specificity (Koch et al., 1983, PMID: 6192569).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: MHC I (MHC, Class I Products)

Background: R1.9.6, H-2, MHC-I, MHC Class I