Recombinant MHC Class II antibody
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- Target See all MHC Class II (MHC2) Antibodies
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Mouse, Rat
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Host
- Rabbit
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Clonality
- Chimeric
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Conjugate
- This MHC Class II antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Purpose
- Anti-MHC II [P7/7], Rabbit IgG, kappa
- Specificity
- This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.
- Cross-Reactivity
- Mouse
- Characteristics
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Original Species of Ab: Rat
Original Format of Ab: IgG2b
- Purification
- Protein A affinity purified
- Immunogen
- This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
- Clone
- P7-7
- Isotype
- IgG kappa
- Top Product
- Discover our top product MHC2 Primary Antibody
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- Application Notes
- This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987, Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004, Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).
- Comment
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
- Alternative Name
- MHC II (MHC2 Products)
- Background
- P7.7, I-A, Ia, I-E, MHC-II, MHC Class II
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