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Recombinant MHC Class II antibody

MHC2 Reactivity: Mouse, Rat FACS, IHC, IP Host: Rabbit Chimeric P7-7 unconjugated Recombinant Antibody
Catalog No. ABIN7072422
  • Target See all MHC Class II (MHC2) Antibodies
    MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 48
    • 45
    • 15
    • 9
    • 9
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Mouse, Rat
    Host
    • 46
    • 28
    • 24
    Rabbit
    Clonality
    • 78
    • 19
    • 1
    Chimeric
    Conjugate
    • 31
    • 15
    • 10
    • 10
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MHC Class II antibody is un-conjugated
    Application
    • 70
    • 42
    • 22
    • 22
    • 20
    • 13
    • 13
    • 9
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Purpose
    Anti-MHC II [P7/7], Rabbit IgG, kappa
    Specificity
    This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.
    Cross-Reactivity
    Mouse
    Characteristics

    Original Species of Ab: Rat

    Original Format of Ab: IgG2b

    Purification
    Protein A affinity purified
    Immunogen
    This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
    Clone
    P7-7
    Isotype
    IgG kappa
    Top Product
    Discover our top product MHC2 Primary Antibody
  • Application Notes
    This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987, Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004, Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).
    Comment

    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
    Alternative Name
    MHC II (MHC2 Products)
    Background
    P7.7, I-A, Ia, I-E, MHC-II, MHC Class II
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