Recombinant Desmosome/hemidesmosome antibody
Reactivity: Human
IEM, IF
Host: Rabbit
Chimeric
F12
unconjugated
Recombinant Antibody
-
- Target
- Desmosome/hemidesmosome
- Antibody Type
- Recombinant Antibody
- Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Chimeric
-
Conjugate
- Un-conjugated
- Application
- Immunoelectron Microscopy (IEM), Immunofluorescence (IF)
- Purpose
- Anti-desmosome/hemidesmosome [F12], Rabbit IgG, kappa
- Specificity
- F12 recognizes an unknown polypeptide of desmosomal and hemidesmosomal plaques with a MW of 185 kDa (determined from immunoblotting using bovine tongue epithelium extract as the substrate), and by IEM (immunoelectron microscopy) it was determined that it is the intracellular part of the desmosome that is bound (Gilbert, 1997). F12 shares some immunochemical properties with autoantibodies present in paraneoplastic pemphigus sera. Pemphigus diseases are those resulting in blistering of the skin due to the production of autoantibodies against keratinocyte surface proteins - autoantibodies of patients with pemphius vulgaris recognize desmoglein 1 (Dsg1), a major glycoprotein of the desmosome complex.
- Characteristics
-
Original Species of Ab: Human
Original Format of Ab: IgM
- Purification
- Protein A affinity purified
- Immunogen
- F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line.
- Clone
- F12
- Isotype
- IgG kappa
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-
- Application Notes
- F12 labels the keratinocyte membrane and the basement membrane zone of rat tongue sections by indirect immunofluorescence (IIF). The antibody also stains both the cell membrane and the basement membrane zone of stratified squamous epithelia, and other epithelial tissues such as urinary bladder, small bowel, thymus, and liver. Non-epithelial tissues, such as myocardium are additionally stained. Sera from patients with pemphigus vulgaris can be incubated with human skin sections to determine their capacity to block the binding of F12 in an IIF blocking assay. IEM (immunoelectron microscopy) can be used to determine the ultralocalization of the antibody.
- Comment
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
-
- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
- Target
- Desmosome/hemidesmosome
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