Recombinant CD80 (Galiximab Biosimilar) antibody

Catalog No. ABIN7072388

Product Details anti-CD80 (Galiximab Biosimilar) Antibody

Target: CD80 (Galiximab Biosimilar)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD80 (Galiximab Biosimilar) antibody is un-conjugated

Application: Blocking Reagent (BR), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Purpose: Anti-CD80 [IDEC-114 (Galiximab)], Rabbit IgG, lambda

Specificity: Galiximab is a primatized mAb which consists of human constant and primate (cynomolgus macaque) variable regions and binds specifically to CD80. CD80 is a surface glycoprotein and a member of the B7 family of costimulatory molecules. CD80 antigen regulates T cell activation (through interacting with CD28 or CD152) and is expressed transiently in antigen-presenting cells, T cells and normal B cells, and expressed consitutively on various subtypes of B-cell lymphomas.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)

Clone: IDEC-114

Isotype: IgG lambda

Application Details

Application Notes: Galiximab acts to decrease in cell proliferation, inhibition of the constitutively active NF-κB pathway, increase in apoptosis and ADCC against various B-cell lymphoma cell lines. Galiximb affects CD28 and CD152 interactions with CD80. This antibody can also be used for FC, WB, ELISA, IP and IHC.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD80 (Galiximab Biosimilar)

Target: CD80 (Galiximab Biosimilar)

Abstract: CD80 (Galiximab Biosimilar) Products

Target Type: Biosimilar

Background: B7-1, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7

UniProt: P33681