Recombinant CD27 antibody

Catalog No. ABIN7072375

Product Details anti-CD27 Antibody

Target: CD27 (CD27 Molecule (CD27))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Activation (Act)

Purpose: Anti-CD27 [LG.3A10], Rabbit IgG, kappa

Specificity: This antibody is specific for mouse CD27, which, as in the human system, is largely restricted to cells of the T cell lineage, and is expressed on the great majority of both αβ and γδ T lymphocytes, and thymocytes.

Cross-Reactivity: Human, Rat

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: This antibody was produced by injecting Armenian hamsters intraperitoneally with a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530) in Freund's adjuvant

Clone: LG-3A10

Isotype: IgG kappa

Application Details

Application Notes: This antibody is specific for murine CD27, and has been used to assess the distribution of CD27 expression using IF, FC and IP analysis (Gravstein, 1995). Upon cross-linking, this antibody has been shown to induce a 4-fold increase in T cell proliferation in response to suboptimal stimulation with concanavalin A (Gravestein, 1995), indicating that this antibody is able to mimic ligand binding.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27 (CD27 Products)

Background: TNFRS7, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7

UniProt: P41272

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints