Recombinant Integrin beta 7 antibody

Catalog No. ABIN7072355

Product Details anti-Integrin beta 7 Antibody

Target: Integrin beta 7 (ITGB7)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This Integrin beta 7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Purpose: Anti-Integrin beta-7 [FIB27], Rabbit IgG, kappa

Specificity: FIB27 binds specifically to both human and mouse integrin beta-7 at epitope region Dl on which is involved in all a4b7-mediated adhesion events. Does not compete with DAKT32 for binding. FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-a4B7 antibody and induces aggregation via an a4B7-dependent pathway). Integrin beta-7 is one subunit is a heterodimer which makes up an intergin molecule. In the mouse beta-7 is selectively found on the majority of mature lymphocytes, whereas a small subpopulation of thymocytes and bone marrow cells express beta-7. Beta-7 is also expressed in variable amounts in CD4 T memory cells. Integrin molecules mediate cell-cell and cell-extracellular matrix adhesion and are involved in lymphocyte homing, leukocyte recruitment to inflammatory sites, myogenesis, hemopoiesis, and melanoma metastasis.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: FIB27 was prepared by immunizing rats with TK1 cells.

Clone: FIB27

Isotype: IgG kappa

Application Details

Application Notes: FIB27 can be used for FC, IP and can block TKI cell aggregation and beta-7 intergrin-mediated cell adhesion.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Integrin beta 7

Target: Integrin beta 7 (ITGB7)

Alternative Name: Integrin beta-7 (ITGB7 Products)

Background: FIB-27, FIB 27, Fib-27, Fib27, Fib 27, fib27, fib-27, fib 27, Integrin b7, integrin b-7, ITGB7, Gut homing receptor beta subunit

Pathways: Integrin Complex