SNTA1 antibody (Intracellular)

Catalog No. ABIN7043797

This Rabbit Polyclonal antibody specifically detects SNTA1 in WB, IF and IHC. It exhibits reactivity toward Mouse.

Quick Overview for SNTA1 antibody (Intracellular) (ABIN7043797)

Target: SNTA1 (Syntrophin, alpha 1 (Dystrophin-Associated Protein A1, 59kDa, Acidic Component) (SNTA1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNTA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-SNTA1 Antibody

Binding Specificity: AA 191-205, Intracellular

Purpose: A Rabbit Polyclonal Antibody to α1-Syntrophin (SNTA1)

Specificity: Intracellular, PH1 domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-alpha1-Syntrophin (SNTA1) Antibody is directed against an epitope of mouse syntrophin alpha 1. Anti-α1-Syntrophin (SNTA1) Antibody (ABIN7043797, ABIN7045164 and ABIN7045165) can be used in western blot and immunohistochemistry applications. It has been designed to recognize syntrophin alpha 1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RQPSSPGPQPRNLSE, corresponding to amino acid residues 191-205 of mouse alpha1-syntrophin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7237350)

Blocking Peptide: (ABIN7237350)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SNTA1

Target: SNTA1 (Syntrophin, alpha 1 (Dystrophin-Associated Protein A1, 59kDa, Acidic Component) (SNTA1))

Alternative Name: SNTA1

Background: Syntrophin alpha 1, Syntrophin-1, 59 kDa dystrophin-associated protein A1 acidic component 1,α1-Syntrophin is a component of the dystrophin scaffold-protein complex that acts in recruiting proteins through their multiple protein-protein interaction motifs to the cytoplasmic side of plasma membranes. Proteins interacting with α1-syntrophin include voltage-gated sodium channels, neuronal nitric oxide synthase, calmodulin, ankyrin repeat-rich membrane spanning protein, G-proteins and more. The syntrophin family comprises five members: α1-syntrophin, β1-syntrophin, β2-syntrophin, γ1-syntrophin, and γ2-syntrophin1.α1-Syntrophin is a peripheral cytoplasmic membrane protein in skeletal and cardiac muscle cells1.The α1-syntrophin structure contains four conserved domains, two pleckstrin homology domains (PH1 and PH2), a PSD95-disks large-ZO1 (PDZ) domain and a syntrophin unique (SU) COOH-terminal domain2. The pleckstrin domains are responsible for the recruitment of proteins to the sarcolemma. The PDZ domain is inserted within the PH1 domain and binds to NOS-1 in skeletal muscle. This domain also binds to the C-terminus of the Kir2.1 channel and also to NaV1.5 and so allowing them to interact. The SU domain binds syntrophin to dystrophin2,3. α1-Syntrophin is also responsible for the site-specific anchoring of AQP4 protein. α1-Syntrophin deletion leads to the disruption of the polarized subcellular expression of AQP4 in the perivascular membranes4. Several studies have shown that syntrophin-deficiency in Duchenne muscular dystrophy can ultimately lead to muscle degeneration. Syntrophin-deficiency was also shown to reduce levels of nNOS in mice5,6.

Alternative names: alpha1-Syntrophin (SNTA1), Syntrophin alpha 1, Syntrophin-1, 59 kDa dystrophin-associated protein A1 acidic component 1

Gene ID: 20648

NCBI Accession: NM_003098

UniProt: Q61234

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development