Nerve Growth Factor antibody (Pro-Domain)

Catalog No. ABIN7043556

Product Details anti-Nerve Growth Factor Antibody

Target: Nerve Growth Factor (NGF)

Binding Specificity: AA 84-104, Pro-Domain

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity (Details): The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.

Characteristics: Anti-proNGF Antibody is directed against rat proNGF. Anti-proNGF Antibody (ABIN7043556, ABIN7044751 and ABIN7044752)) can be used in western blot and immunohistochemical applications. The antibody specifically reacts with proNGF. It has been designed to recognize proNGF from mouse, rat and human samples. \nRelated products for neutralizing proNGF activity: \nMouse NGF/proNGF Neutralizing Antibody (#) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: proNGF (NGF Products)

Synonyms: Beta-NGF antibody, HSAN5 antibody, NGFB antibody, Ngfb antibody, ngfb antibody, ns:zft386 antibody, xx:zft386 antibody, zgc:109730 antibody, beta-NGF antibody, hsan5 antibody, beta-ngf antibody, NGF antibody, ngf antibody, nerve growth factor antibody, nerve growth factor b (beta polypeptide) antibody, nerve growth factor S homeolog antibody, NGF antibody, Ngf antibody, ngfb antibody, ngf antibody, ngf.S antibody

Background: Alternative names: proNGF, Nerve growth factor precursor

Gene ID: 310738

NCBI Accession: NM_002506

UniProt: P25427

Pathways: Regulation of Cell Size