EPH Receptor B4 antibody (Extracellular, N-Term)
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- Target See all EPH Receptor B4 (EPHB4) Antibodies
- EPH Receptor B4 (EPHB4)
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Binding Specificity
- AA 472-486, Extracellular, N-Term
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This EPH Receptor B4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Characteristics
- Anti-EphB4 (extracellular) Antibody (ABIN7043127, ABIN7044253 and ABIN7044254)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize EphB4 from rat, mouse, and human samples.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)HEKGAEGPSSVRFLK, corresponding to amino acid residues 472-486 of mouse EphB4
- Isotype
- IgG
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
- Concentration
- 0.8 mg/mL
- Buffer
- Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- EPH Receptor B4 (EPHB4)
- Alternative Name
- EphB4 (EPHB4 Products)
- Background
- Alternative names: EphB4, Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK
- Gene ID
- 13846
- NCBI Accession
- NM_004444
- UniProt
- P54761
- Pathways
- RTK Signaling
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