Angiotensin II Type 2 Receptor antibody (Extracellular, N-Term) (Atto 488)

Catalog No. ABIN7042926

Product Details anti-Angiotensin II Type 2 Receptor Antibody (Atto 488)

Target: Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))

Binding Specificity: AA 21-35, Extracellular, N-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiotensin II Type 2 Receptor antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Live Cell Imaging (LCI)

Cross-Reactivity (Details): Will not recognize human AT2 Receptor.

Characteristics: Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (ABIN7042925, ABIN7043902 and ABIN7043903)) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AT2 receptor from rat and mouse samples. The antibody won't recognize human AT2R. \nAnti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody (#ABIN7042926) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody has been tested in live cell imaging and immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Angiotensin II Type 2 Receptor

Target: Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))

Alternative Name: Angiotensin II Receptor Type-2 (AGTR2 Products)

Synonyms: AT2 antibody, ATGR2 antibody, MRX88 antibody, AI316812 antibody, AW107640 antibody, AT2-R antibody, AT2R antibody, AT₂R antibody, at2 antibody, zgc:113016 antibody, AGTR2 antibody, angiotensin II receptor type 2 antibody, angiotensin II receptor, type 2 antibody, AGTR2 antibody, Agtr2 antibody, agtr2 antibody

Background: Alternative names: Angiotensin II Receptor Type-2, AT2 Receptor, AT2R, AGTR2, MRX88

Gene ID: 24182

NCBI Accession: NM_000686

UniProt: P35351

Pathways: ACE Inhibitor Pathway, Hormone Transport, Regulation of Systemic Arterial Blood Pressure by Hormones