ADRB3 antibody (2nd Extracellular Loop, Cys186)

Catalog No. ABIN7042907

Product Details anti-ADRB3 Antibody

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Binding Specificity: 2nd Extracellular Loop, AA 180-192, Cys186

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Anti-beta3-Adrenergic Receptor (extracellular) Antibody is directed against an extracellular epitope of the mouse β3-adrenoceptor. Anti-β3-Adrenergic Receptor (extracellular) Antibody (ABIN7042907, ABIN7043912 and ABIN7043913)) can be used in western blot and immunohistochemical applications. It has been designed to recognize both splice variants of the β3AR from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: DAEAQESH*SNPRC, corresponding to amino acid residues 180-192 of mouse ADRB3 with replacement of cysteine 186 (C186) with serine (*S)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.75 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADRB3

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Alternative Name: beta3-Adrenergic Receptor (ADRB3 Products)

Synonyms: BETA3AR antibody, Adrb-3 antibody, ADRB antibody, BAR3 antibody, B3AR antibody, GPCR antibody, im:7154935 antibody, si:ch211-215f19.1 antibody, zgc:194843 antibody, zgc:194852 antibody, adrenoceptor beta 3 antibody, adrenergic receptor, beta 3 antibody, adrenoceptor beta 3a antibody, adrenoceptor beta 3b antibody, ADRB3 antibody, Adrb3 antibody, adrb3a antibody, adrb3b antibody

Background: Alternative names: beta3-Adrenergic Receptor, Beta-3 adrenoceptor, Beta-3 adrenoreceptor, ADRB3, B3AR

Gene ID: 11556

NCBI Accession: NM_000025

UniProt: P25962

Pathways: cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Brown Fat Cell Differentiation