The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.
Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Immunogen
RBL-2H3 cells expressing human CLEC9A fused to an HA epitope
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
9830005G06Rik antibody, DNGR-1 antibody, DNGR1 antibody, UNQ9341 antibody, RGD1562513 antibody, C-type lectin domain family 9, member a antibody, C-type lectin domain containing 9A antibody, C-type lectin domain family 9, member A antibody, Clec9a antibody, CLEC9A antibody
Background
C-type lectin domain containing 9A,CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.,CLEC9A, DNGR1, DNGR-1, UNQ9341