CD77 antibody

Catalog No. ABIN7013943

Product Details anti-CD77 Antibody

Target: CD77

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD77 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Immunogen: Daudi cell line (Burkitt´s lymphoma)

Clone: 38-13

Isotype: IgM

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD77

Target: CD77

Abstract: CD77 Products

Background: CD77 (globotriaosylceramide Gb3), also known as the Pk blood group antigen, BLA (Burkitt´s lymphoma associated antigen), or CTH (ceramide trihexoside) is a neutral glycosphingolipid composed of three carbohydrate molecules linked to a lipid moiety in the cell membrane (Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer). It is expressed on germinal center B cells, Burkitt´s lymphoma cells, it can be induced on extrafolicular B cells and it is also found on endothelia and epithelia. CD77 may be involved in elimination of germinal center B cells that fail to produce high affinity antibodies, and serves also as receptor for shiga toxin and verotoxin.,globotriaosylceramide Gb3, Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer, P blood group