CD8B antibody

Catalog No. ABIN7013937

Product Details anti-CD8B Antibody

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.

Purification: Purified by protein-A affinity chromatography.

Endotoxin Level: Low Endotoxin

Immunogen: CD8 positive Wistar rat splenic T cell hybridomas

Clone: 341

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Functional application: In vitro stimulation, in vivo depletion.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8b (CD8B Products)

Synonyms: Cd8b antibody, Ly-3 antibody, Ly-C antibody, Lyt-3 antibody, CD8B1 antibody, LEU2 antibody, LY3 antibody, LYT3 antibody, P37 antibody, Cd8b1 antibody, CD8b molecule antibody, CD8 antigen, beta chain 1 antibody, Cd8b antibody, CD8B antibody, Cd8b1 antibody

Background: CD8b molecule,The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,Ly-3, Ly-C, Lyt-3

Gene ID: 926

UniProt: P10966

Pathways: TCR Signaling