Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HLA-F antibody (PE)

HLAF Reactivity: Human FACS Host: Mouse Monoclonal 3D11 PE
Catalog No. ABIN7013919
  • Target See all HLA-F (HLAF) Antibodies
    HLA-F (HLAF) (HLA Class I alpha F (HLAF))
    Reactivity
    • 17
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 15
    • 2
    Mouse
    Clonality
    • 14
    • 3
    Monoclonal
    Conjugate
    • 14
    • 1
    • 1
    • 1
    This HLA-F antibody is conjugated to PE
    Application
    • 13
    • 6
    • 4
    • 2
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody 3D11 recognizes an extracellular epitope of HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.
    Purification
    Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Inclusion body-derived HLA-F heavy chain
    Clone
    3D11
    Isotype
    IgG1
  • Application Notes
    Flow cytometry: Recommended dilution: 1-5 μg/mL, extracellular and intracellular staining.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Target
    HLA-F (HLAF) (HLA Class I alpha F (HLAF))
    Alternative Name
    HLA-F (HLAF Products)
    Synonyms
    HLAF antibody, CDA12 antibody, HLA-5.4 antibody, HLA-CDA12 antibody, major histocompatibility complex, class I, F antibody, HLA-F antibody
    Background
    Major histocompatibility complex, class I, F,HLA-F, an MHC class I molecule, is a type I transmembrane protein (heavy chain), which forms heterodimers with beta-2 microglobulin (light chain) and binds to KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, and TAP. Unlike most other HLA proteins, HLA-F is mainly localized in the endoplasmic reticulum and Golgi apparatus, with only a small amount present on the cell surface in some cell types, surface expression can be induced by cell activation. It is thought to bind a restricted subset of peptides for immune presentation. Multiple transcript variants encoding different isoforms have been found for HLA-F gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.,CDA12
    Gene ID
    3134
    UniProt
    P30511
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
You are here:
Support