HLA-F antibody

Catalog No. ABIN7013918

Product Details anti-HLA-F Antibody

Target: HLA-F (HLAF) (HLA Class I alpha F (HLAF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-F antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody 3D11 recognizes an extracellular epitope of HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.

Purification: Purified by protein-A affinity chromatography.

Immunogen: Inclusion body-derived HLA-F heavy chain

Clone: 3D11

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL, extracellular and intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for HLA-F

Target: HLA-F (HLAF) (HLA Class I alpha F (HLAF))

Alternative Name: HLA-F (HLAF Products)

Synonyms: HLAF antibody, CDA12 antibody, HLA-5.4 antibody, HLA-CDA12 antibody, major histocompatibility complex, class I, F antibody, HLA-F antibody

Background: Major histocompatibility complex, class I, F,HLA-F, an MHC class I molecule, is a type I transmembrane protein (heavy chain), which forms heterodimers with beta-2 microglobulin (light chain) and binds to KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, and TAP. Unlike most other HLA proteins, HLA-F is mainly localized in the endoplasmic reticulum and Golgi apparatus, with only a small amount present on the cell surface in some cell types, surface expression can be induced by cell activation. It is thought to bind a restricted subset of peptides for immune presentation. Multiple transcript variants encoding different isoforms have been found for HLA-F gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.,CDA12

Gene ID: 3134

UniProt: P30511

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response