EPH Receptor B6 antibody (Internal Region)

Catalog No. ABIN7013872

Product Details anti-EPH Receptor B6 Antibody

Target: EPH Receptor B6 (EPHB6)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EPH Receptor B6 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: EPHB6

Sequence: EVIAHGKHTT SSD

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine and Brain (Cerebellum). Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary testing showed a band at approx 80 kDa in U251 cell lysate and approx.75 kDa in Human Pancreas lysate after 1-2 μg/mL antibody staining (calculated MW of 79.8 kDa according to NP_001267723.2). Primary incubation 1 hour at room tem

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended c

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EPH Receptor B6

Target: EPH Receptor B6 (EPHB6)

Alternative Name: EPHB6 (EPHB6 Products)

Synonyms: CEK9 antibody, EPHB5 antibody, Cekl antibody, Mep antibody, HEP antibody, EPH receptor B6 antibody, Eph receptor B6 antibody, EPHB6 antibody, Ephb6 antibody

Background: EPHB6, EPH receptor B6, HEP, MGC129910, MGC129911, ephrin receptor EphB6

Gene ID: 2051, 13848, 312275

Pathways: RTK Signaling, Hormone Transport