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EDF1 antibody (N-Term)

Verified EDF1 Reactivity: Human, Mouse WB, IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN7013866
  • Target See all EDF1 Antibodies
    EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))
    Binding Specificity
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 16
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    Human, Mouse
    Host
    • 15
    • 2
    • 1
    Goat
    Clonality
    • 17
    • 1
    Polyclonal
    Conjugate
    • 18
    This EDF1 antibody is un-conjugated
    Application
    • 18
    • 8
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    EDF1 / MBF1
    Sequence
    AESDWDTVTV LRK
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Top Product
    Discover our top product EDF1 Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 19 kDa band observed in nuckear lysates of cell line HEK293, approx. 18 kDa in nuckear lysates of cell line HeLa, and approx 17 kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4 kDa according to Human NP_003783.1 and Mous

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

    Restrictions
    For Research Use only
  • Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))
    Alternative Name
    EDF1 (EDF1 Products)
    Synonyms
    edf-1 antibody, mbf1 antibody, EDF-1 antibody, MBF1 antibody, 0610008L11Rik antibody, AA409425 antibody, CAP-19 antibody, zgc:73192 antibody, zgc:86829 antibody, endothelial differentiation related factor 1 S homeolog antibody, endothelial differentiation related factor 1 antibody, endothelial differentiation-related factor 1 antibody, Mbf1p antibody, edf1.S antibody, EDF1 antibody, Edf1 antibody, edf1 antibody, MBF1 antibody
    Background
    EDF1, MBF1, EDF-1, MGC9058, endothelial differentiation-related factor 1, multiprotein bridging factor 1
    Gene ID
    8721, 59022
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