SCRIB antibody (Internal Region)

Catalog No. ABIN7013862

Product Details anti-SCRIB Antibody

Target: SCRIB (Scribbled Homolog (SCRIB))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SCRIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: SCRIB / Scribble

Sequence: PEGPGKEKEL PGQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_874365.3, NP_056171.3).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Application Details

Application Notes:

Western Blot: Approx 200-250 kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178 kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary inc

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SCRIB

Target: SCRIB (Scribbled Homolog (SCRIB))

Alternative Name: SCRIB (SCRIB Products)

Background: SCRIB, scribbled homolog (Drosophila), CRIB1, SCRB1, SCRIB1, Vartul, scribble

Gene ID: 23513

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Tube Formation, Synaptic Vesicle Exocytosis, Asymmetric Protein Localization