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POLR2A/RPB1 antibody (pSer5)

Name: POLR2A/RPB1 antibody (pSer5)
Brand: antibodies-online
SKU: ABIN7265278
Price: 590 USD
Availability: InStock

POLR2A Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts of MCF7 cells using Phospho-POLR2A(S5) Polyclonal Antibody at dilution of 1:1000. (POLR2A/RPB1 antibody (pSer5))

Immunofluorescence analysis of MCF7 cells using Phospho-POLR2A(S5) Polyclonal Antibody at dilution of 1:100. (POLR2A/RPB1 antibody (pSer5))

Immunohistochemistry of paraffin-embedded Rat testis using Phospho-POLR2A(S5) Polyclonal Antibody at dilution of 1:100 (40x lens). (POLR2A/RPB1 antibody (pSer5))

IHC (p)

Immunohistochemistry of paraffin-embedded Human colon using Phospho-POLR2A(S5) Polyclonal Antibody at dilution of 1:100 (40x lens). (POLR2A/RPB1 antibody (pSer5))

IHC (p)

Immunohistochemistry of paraffin-embedded Mouse liver using Phospho-POLR2A(S5) Polyclonal Antibody at dilution of 1:100 (40x lens). (POLR2A/RPB1 antibody (pSer5))

IHC (p)

5 images

Catalog No. ABIN7265278

Datasheet as PDF

Target See all POLR2A/RPB1 (POLR2A) Antibodies

POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
Binding Specificity
16

16

11

7

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer5
Reactivity
88

46

38

6

6

6

5

5

4

3

3

2

2

1

1
Human, Mouse, Rat
Host
71

17

1

1

1
Rabbit
Compatible Secondaries
Clonality
55

36
Polyclonal
Conjugate
53

6

5

5

5

5

5

5

1

1
This POLR2A/RPB1 antibody is un-conjugated
Application
47

25

24

20

12

10

8

7

3

3

3

2

2

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics

Phosphorylated antibody

Purification

Affinity purification

Immunogen

A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A

Isotype

IgG
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibody
POLR2A Reactivity: Human WB, IHC, IF, IP, ChIP Host: Rabbit Polyclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibody
POLR2A Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibody
POLR2A Reactivity: Human WB, ELISA, ChIP, FM, CUT&RUN Host: Mouse Monoclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibody
POLR2A Reactivity: Human WB, ELISA, ChIP, FM, CUT&RUN Host: Mouse Monoclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibody
POLR2A Reactivity: Human WB, IHC, ELISA, IF, IP Host: Rabbit Monoclonal 2G1 unconjugated Recombinant Antibody

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 128-258) antibody
POLR2A Reactivity: Human ELISA, FACS Host: Mouse Monoclonal 1B1D8 unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 76-298) antibody
POLR2A Reactivity: Human IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibody
POLR2A Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Monoclonal 2H4 unconjugated Recombinant Antibody

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (Internal Region) antibody
POLR2A Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibody
POLR2A Reactivity: Human, Saccharomyces cerevisiae WB, IHC, IF, FACS, StM Host: Mouse Monoclonal CTD 8A7 unconjugated

Application Notes

WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:100

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C. Avoid freeze / thaw cycles.
Target

POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name

POLR2A (POLR2A Products)

Synonyms

NCU02103.1 antibody, 19.m02399 antibody, POLR2A antibody, 220kDa antibody, Rpb1 antibody, Rpo2-1 antibody, POLR2 antibody, POLRA antibody, RPB1 antibody, RPBh1 antibody, RPO2 antibody, RPOL2 antibody, RpIILS antibody, hRPB220 antibody, hsRPB1 antibody, DNA-directed RNA polymerase II largest subunit antibody, DNA-directed RNA polymerase II subunit RPB1 antibody, RNA polymerase II large subunit Rpb1 antibody, RNA polymerase II subunit A antibody, DNA-directed RNA polymerase II largest subunit, putative antibody, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody, polymerase (RNA) II (DNA directed) polypeptide A antibody, NCU02103 antibody, BBOV_I003360 antibody, EDI_169920 antibody, CMU_000800 antibody, SJAG_01713 antibody, MGYG_01527 antibody, POLR2A antibody, TP03_0185 antibody, EBI_22514 antibody, CGB_E5020W antibody, polr2a antibody, Polr2a antibody

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Molecular Weight

Observed_MW: 260 kDa
Calculated_MW: 63 kDa/217 kDa

Gene ID

5430

UniProt

P24928

Pathways

Regulatory RNA Pathways