HIRA antibody

Catalog No. ABIN7264499

Product Details anti-HIRA Antibody

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIRA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human HIRA (NP_003316.3).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: HIRA (HIRA Products)

Background: This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Molecular Weight:

Observed_MW: 112 kDa

Calculated_MW: 89 kDa/111 kDa

Gene ID: 7290

UniProt: P54198

Pathways: Chromatin Binding