Topoisomerase I antibody

Catalog No. ABIN7011954

Product Details anti-Topoisomerase I Antibody

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: A synthetic peptide of human TOP1 (NP_003277.1).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1 (TOP1 Products)

Target Type: Viral Protein

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Molecular Weight:

Observed_MW: 108 kDa

Calculated_MW: 90 kDa

Gene ID: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance