RAD50 antibody

Catalog No. ABIN7010313

Product Details anti-RAD50 Antibody

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: A synthetic peptide of human RAD50 (NP_005723.2).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Alternative Name: RAD50 (RAD50 Products)

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Molecular Weight:

Observed_MW: 170 kDa

Calculated_MW: 138 kDa/153 kDa/154 kDa

Gene ID: 10111

UniProt: Q92878

Pathways: DNA Damage Repair, Protein targeting to Nucleus