POLH antibody

Catalog No. ABIN7259837

Product Details anti-POLH Antibody

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human DNA polymerase eta (NP_006493.1).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for POLH

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Alternative Name: DNA polymerase eta (POLH Products)

Background: This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight:

Observed_MW: 70 kDa

Calculated_MW: 46 kDa/78 kDa

Gene ID: 5429

UniProt: Q9Y253

Pathways: DNA Damage Repair