POLR2E antibody

Catalog No. ABIN7009701

Product Details anti-POLR2E Antibody

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human POLR2E (NP_002686.2).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for POLR2E

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Alternative Name: POLR2E (POLR2E Products)

Synonyms: MGC68604 antibody, polr2e antibody, wu:fk97g10 antibody, zgc:101098 antibody, 2410021N14Rik antibody, 25kDa antibody, AW208866 antibody, RPB5 antibody, XAP4 antibody, RPABC1 antibody, hRPB25 antibody, hsRPB5 antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog antibody, RNA polymerase II subunit E antibody, polymerase (RNA) II (DNA directed) polypeptide E, b antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa antibody, polymerase (RNA) II (DNA directed) polypeptide E antibody, DNA-directed RNA polymerases I, II, and III subunit RPABC1 antibody, polr2e.S antibody, POLR2E antibody, polr2eb antibody, polr2e antibody, Polr2e antibody, rpb-5 antibody

Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight:

Observed_MW: 26 kDa

Calculated_MW: 24 kDa

Gene ID: 5434

UniProt: P19388

Pathways: Regulatory RNA Pathways