AMPK alpha antibody

Catalog No. ABIN7259664

Product Details anti-AMPK alpha Antibody

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMPK alpha antibody is un-conjugated

Application: Immunofluorescence (IF)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant protein of human PRKAA1/PRKAA2.

Isotype: IgG

Application Details

Application Notes: IF 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: PRKAA1/PRKAA2 (SNF1A Products)

Synonyms: AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, PRKAA2 antibody

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed./The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Gene ID: 5562, 5563

UniProt: Q13131, P54646

Pathways: Warburg Effect