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RPS6 antibody (AA 151-249)

Name: RPS6 antibody (AA 151-249)
SKU: ABIN700885
Price: 384.62 USD
Availability: InStock

RPS6 Reactivity: Mouse, Rat WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with RPS6 Polyclonal Antibody (bs-3565R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (RPS6 antibody (AA 151-249))

IHC (p)

Antigen: 0. (RPS6 antibody (AA 151-249))

ELISA

Lane 1: mouse brain lysates Lane 2: mouse liver lysates probed with Anti RPS6Polyclonal Antibody, Unconjugated (ABIN700885) at 1:200 in 4 °C. (RPS6 antibody (AA 151-249))

SDS

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with RPS6 Polyclonal Antibody (bs-3565R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (RPS6 antibody (AA 151-249))

IHC (p)

4 images

Catalog No. ABIN700885

Datasheet as PDF

Target See all RPS6 Antibodies

RPS6 (Ribosomal Protein S6 (RPS6))
Binding Specificity
45

25

20

20

14

14

13

9

9

6

5

5

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 151-249
Reactivity
128

113

101

12

12

10

10

9

8

8

7

7

5

5

5

4

2

2

2

1
Mouse, Rat
Host
145

10

2

2
Rabbit
Compatible Secondaries
Clonality
145

14
Polyclonal
Conjugate
83

9

8

6

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2
This RPS6 antibody is un-conjugated
Application
133

56

39

39

38

32

29

18

11

10

9

3

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Mouse, Rat

Predicted Reactivity

Human,Cow,Pig,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human RPS6

Isotype

IgG

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RPS6 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ribosomal Protein S6 (RPS6) antibody
RPS6 Reactivity: Human, Rat WB, ELISA, IHC, IF, FACS Host: Mouse Monoclonal 981CT2-1-1 unconjugated

anti-Ribosomal Protein S6 (RPS6) (Thr826) antibody
RPS6 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ribosomal Protein S6 (RPS6) (AA 13-52), (N-Term) antibody
RPS6 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Ribosomal Protein S6 (RPS6) (AA 220-249) antibody
RPS6 Reactivity: Human WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated

anti-Ribosomal Protein S6 (RPS6) (AA 227-257) antibody
RPS6 Reactivity: Human, Mouse WB, IHC (p), FACS, EIA Host: Rabbit Polyclonal unconjugated

anti-Ribosomal Protein S6 (RPS6) (AA 35-229) antibody
RPS6 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ribosomal Protein S6 (RPS6) (pSer235), (pSer236) antibody
RPS6 Reactivity: Mouse, Rat WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ribosomal Protein S6 (RPS6) (pSer235) antibody
RPS6 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name

RPS6 (RPS6 Products)

Background

Synonyms: S6, 40S ribosomal protein S6, Phosphoprotein NP33, RPS6, OK/SW-cl.2
Background: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

Gene ID

6194

UniProt

P62753

Pathways

Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly