EIF3A antibody

Catalog No. ABIN7254935

Product Details anti-EIF3A Antibody

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human EIF3A (NP_003741.1).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for EIF3A

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Alternative Name: EIF3A (EIF3A Products)

Synonyms: EIF3 antibody, EIF3S10 antibody, P167 antibody, TIF32 antibody, eIF3-p170 antibody, eIF3-theta antibody, p180 antibody, p185 antibody, EIF6 antibody, A830012B05Rik antibody, Csma antibody, Eif3 antibody, Eif3s10 antibody, mKIAA0139 antibody, ZH12 antibody, eif3s10 antibody, eIF3 p110 antibody, eIF3a antibody, eukaryotic translation initiation factor 3 subunit A antibody, eukaryotic translation initiation factor 3, subunit A antibody, eukaryotic translation initiation factor 3 subunit A S homeolog antibody, translation initiation factor eIF3a antibody, EIF3A antibody, Eif3a antibody, eif3a.S antibody, tif32 antibody

Background: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Molecular Weight:

Observed_MW: 180 kDa

Calculated_MW: 162 kDa/166 kDa

Gene ID: 8661

UniProt: Q14152

Pathways: Ribonucleoprotein Complex Subunit Organization