RCC1 antibody (AA 11-120)

Catalog No. ABIN700585

Product Details anti-RCC1 Antibody

Target: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Binding Specificity: AA 11-120

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCC1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RCC1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RCC1

Target: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Alternative Name: RCC1 (RCC1 Products)

Background:

Synonyms: Cell cycle regulatory protein, CHC 1, CHC1, Chromosome condensation 1, Chromosome condensation protein 1, Guanine nucleotide releasing protein, Ran GEF, RanGEF, RCC 1, RCC1 I, Regulator of chromosome condensation 1, Regulator of chromosome condensation, SHEP1.

Background: Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis. The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes.

Gene ID: 1104

UniProt: P18754

Pathways: Chromatin Binding, Protein targeting to Nucleus