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MBP Tag antibody

WB Host: Mouse Monoclonal 8K2 unconjugated
Catalog No. ABIN7250836
  • Target
    MBP Tag
    Host
    • 12
    • 5
    Mouse
    Clonality
    • 11
    • 5
    Monoclonal
    Conjugate
    • 15
    • 1
    • 1
    Un-conjugated
    Application
    • 15
    • 8
    • 7
    • 5
    • 3
    • 1
    • 1
    Western Blotting (WB)
    Characteristics
    Tag Antibody
    Purification
    Protein A purification
    Immunogen
    Recombinant Protein
    Clone
    8K2
    Isotype
    IgG
  • Application Notes
    WB 1:5000-1:10000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Avoid freeze / thaw cycles.
  • Target
    MBP Tag
    Alternative Name
    MBP-Tag
    Target Type
    Tag
    Background
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site.
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