RNF146 antibody

Catalog No. ABIN7004025

Product Details anti-RNF146 Antibody

Target: RNF146 (Ring Finger Protein 146 (RNF146))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF146 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Fusion protein of human RNF146

Isotype: IgG

Application Details

Application Notes: IHC 1:30-1:150, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.02 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for RNF146

Target: RNF146 (Ring Finger Protein 146 (RNF146))

Alternative Name: RNF146 (RNF146 Products)

Synonyms: RP3-351K20.1 antibody, dJ351K20.1 antibody, 2610509H23Rik antibody, Iduna antibody, RNF146 antibody, rnf146 antibody, dactylidin antibody, wu:fi29e08 antibody, zgc:55909 antibody, ring finger protein 146 antibody, RING finger protein 146 antibody, ring finger protein 146 S homeolog antibody, RNF146 antibody, Rnf146 antibody, rn146 antibody, rnf146.S antibody, rnf146 antibody

Background: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.

UniProt: Q9NTX7