HLA-DOB antibody

Catalog No. ABIN7245230

Product Details anti-HLA-DOB Antibody

Target: HLA-DOB (Major Histocompatibility Complex, Class II, DO beta (HLA-DOB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DOB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Synthetic peptide of human HLA-DOB

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:30-1:150, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for HLA-DOB

Target: HLA-DOB (Major Histocompatibility Complex, Class II, DO beta (HLA-DOB))

Alternative Name: HLA-DOB (HLA-DOB Products)

Synonyms: DOB antibody, major histocompatibility complex, class II, DO beta antibody, HLA-DOB antibody, PATR-DOB antibody

Background: HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. HLA-DOB (Major Histocompatibility Complex, Class II, DO Beta) is a Protein Coding gene. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and CTLA4 Signaling. GO annotations related to this gene include MHC class II protein complex binding and MHC class II receptor activity. An important paralog of this gene is HLA-DRB1.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 31 kDa

UniProt: P13765

Pathways: Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response