HIRA antibody (AA 251-350)

Catalog No. ABIN700345

Product Details anti-HIRA Antibody

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Binding Specificity: AA 251-350

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIRA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HIRA/DGGR1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: HIRA (HIRA Products)

Background:

Synonyms: DGCR1, DGGR 1, DGGR1, DiGeorge critical region gene 1, HIR, HIR histone cell cycle regulation defective homolog A, HIRA, HIRA protein, HIRA_HUMAN, Protein HIRA, TUP 1, TUP1, TUP1 like enhancer of split protein 1, TUP1-like enhancer of split protein 1, TUPLE 1, TUPLE1.

Background: The HIRA gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in flies, yeast and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Gene ID: 7290

Pathways: Chromatin Binding