HMGCS1 antibody

Catalog No. ABIN7239032

Product Details anti-HMGCS1 Antibody

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Synthetic peptide of human HMGCS1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for HMGCS1

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Alternative Name: HMGCS1 (HMGCS1 Products)

Background: HMG-CoA Synthase exists as both a mitochondrial (mHMGCS) and cytoplasmic (cHMGCS) enzyme that condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA. The HMG-CoA produced by cHMGCS is transformed into mevalonate by HMG-CoA reductase, which starts isoprenoid biosynthesis. End products of the isoprenoid pathway include cholesterol, ubiquinone, dolichol, isopentenyl adenosine and farnesyl groups. mHMGCS, together with HMG-CoA Lyase, is responsible for ketone body biosynthesis. mHMGCS is expressed in liver and kidney. Fasting, cAMP and fatty acids increase the level of transcription of mHMGCS, while feeding and insulin repress it. A regulatory element within the mHMGCS promoter confers transcriptional regulation by PPAR, RXR, COUP-TF and HNF-4.

Molecular Weight: 57 kDa

NCBI Accession: NP_001091742

UniProt: Q01581

Pathways: Regulation of Lipid Metabolism by PPARalpha