BRK1 antibody

Catalog No. ABIN7239023

Product Details anti-BRK1 Antibody

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Synthetic peptide of human BRK1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.6 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for BRK1

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Alternative Name: BRK1 (BRK1 Products)

Background: HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms.

Molecular Weight: 9 kDa

NCBI Accession: NP_060932

UniProt: Q8WUW1

Pathways: RTK Signaling