Separase antibody

Catalog No. ABIN7238939

Product Details anti-Separase Antibody

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Separase antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Synthetic peptide of human ESPL1

Isotype: IgG

Application Details

Application Notes: WB 1:200-1:1000, IHC 1:25-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Separase

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Alternative Name: ESPL1 (ESPL1 Products)

Synonyms: ESP1 antibody, SEPA antibody, mp:zf637-3-001691 antibody, AL024103 antibody, AU045071 antibody, Cerp antibody, PRCE antibody, SSE antibody, extra spindle pole bodies like 1, separase antibody, extra spindle poles like 1 antibody, extra spindle pole bodies 1, separase antibody, ESPL1 antibody, espl1 antibody, Espl1 antibody

Background: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 or STAG2 in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 by ESPL1, or separase, which initiates the final separation of sister chromatids.

Molecular Weight: 233 kDa

NCBI Accession: NP_036423

UniProt: Q14674