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DYKDDDDK Tag antibody

Reactivity: Various Species WB, IP, DB Host: Mouse Monoclonal unconjugated

Catalog No. ABIN7428941

Datasheet as PDF

Target See all DYKDDDDK Tag products

DYKDDDDK Tag
Reactivity
3

1

1
Various Species
Host
53

48

8

6

4
Mouse
Compatible Secondaries
Clonality
61

57
Monoclonal
Conjugate
42

13

9

5

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This DYKDDDDK Tag antibody is un-conjugated
Application
71

58

25

21

16

13

12

12

8

6

4

4

3

3

3

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Purpose

Anti-FLAG Octapeptide (FLAG) Tag Monoclonal Antibody

Specificity

The antibody is a mouse monoclonal antibody raised against FLAG. It has been selected for its ability to recognize FLAG in immunohistochemical staining and western blotting.

Purification

Protein A + Protein G affinity chromatography

Immunogen

OVA Conjugated FLAG Octapeptide (FLAG)
anti-DYKDDDDK Tag antibody
WB, ELISA, IP Host: Rabbit Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF Host: Mouse Monoclonal 2C5 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, IF Host: Mouse Monoclonal 4G5C8 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, DB, ISt Host: Mouse Monoclonal FG4R unconjugated

anti-DYKDDDDK Tag antibody
D Host: Mouse Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
Reactivity: Human WB Host: Rabbit Polyclonal RB0493 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IP Host: Rabbit Polyclonal HRP

anti-DYKDDDDK Tag antibody
IP Host: Mouse Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF Host: Mouse unconjugated

Application Notes

Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells/Immunofluorescence: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions

For Research Use only
Format

Liquid

Buffer

0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date

24 months
Target

DYKDDDDK Tag

Alternative Name

FLAG Octapeptide (DYKDDDDK Tag Products)

Target Type

Tag

Background

FLAG epitope, FLAG-Tag