HIST1H2AB antibody (AA 2-130)

Catalog No. ABIN7438658

Product Details anti-HIST1H2AB Antibody

Target: HIST1H2AB (Histone Cluster 1, H2ab (HIST1H2AB))

Binding Specificity: AA 2-130

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H2AB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Histone Cluster 1, H2ab (HIST1H2AB)

Specificity: The antibody is a rabbit polyclonal antibody raised against HIST1H2AB. It has been selected for its ability to recognize HIST1H2AB in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Histone Cluster 1, H2ab (HIST1H2AB) corresdonding to Ser2~Lys130 (Accession # P04908) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HIST1H2AB

Target: HIST1H2AB (Histone Cluster 1, H2ab (HIST1H2AB))

Alternative Name: Histone Cluster 1, H2ab (HIST1H2AB Products)

Synonyms: H2A/m antibody, H2AFM antibody, H2A.1 antibody, H2a-53 antibody, histone cluster 1 H2A family member b antibody, histone cluster 1, H2ab antibody, HIST1H2AB antibody, Hist1h2ab antibody

Background: HIST1H2AE, H2AFM, H2A/m, H2A Histone Family Member M

Pathways: Telomere Maintenance