T-Bet antibody (AA 241-493)

Catalog No. ABIN7442723

Product Details anti-T-Bet Antibody

Target: T-Bet

Binding Specificity: AA 241-493

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to T-Box Protein 21 (TBX21)

Specificity: The antibody is a rabbit polyclonal antibody raised against TBX21. It has been selected for its ability to recognize TBX21 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant T-Box Protein 21 (TBX21) corresdonding to Leu241~Arg493 (Accession # D3ZCM2)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:300-1200 Immunohistochemistry: 5-20 μg/mL

1:30-120 Immunocytochemistry: 5-20 μg/mL

1:30-120 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for T-Bet

Target: T-Bet

Alternative Name: T-Box Protein 21 (T-Bet Products)

Synonyms: T-PET antibody, T-bet antibody, TBET antibody, TBLYM antibody, TBT1 antibody, Tbet antibody, Tblym antibody, T-box 21 antibody, TBX21 antibody, Tbx21 antibody

Background: TBLYM, TBET, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet