DFFA antibody (AA 51-305)
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- Target See all DFFA Antibodies
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
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Binding Specificity
- AA 51-305
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DFFA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to DNA Fragmentation Factor Subunit Alpha (DFFa)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against DFFa. It has been selected for its ability to recognize DFFa in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant DNA Fragmentation Factor Subunit Alpha (DFFa) corresdonding to Ala51~Ser305 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product DFFA Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- Alternative Name
- DNA Fragmentation Factor Subunit Alpha (DFFA Products)
- Synonyms
- DFF-45 antibody, DFF1 antibody, ICAD antibody, A330085O09Rik antibody, DFF35 antibody, Dff45 antibody, ICAD-L antibody, ICAD-S antibody, DFFA antibody, zgc:92229 antibody, DNA fragmentation factor subunit alpha antibody, DNA fragmentation factor, alpha subunit antibody, DNA fragmentation factor, 45kDa, alpha polypeptide antibody, DNA fragmentation factor, alpha polypeptide antibody, DFFA antibody, Dffa antibody, dffa antibody
- Background
- DFF-45, DFF1, ICAD, DNA Fragmentation Factor,45 kDa,Alpha Polypeptide, Inhibitor of CAD
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
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