TRAF6 antibody (AA 153-423)

Catalog No. ABIN7436348

Product Details anti-TRAF6 Antibody

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Binding Specificity: AA 153-423

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to TNF Receptor Associated Factor 6 (TRAF6)

Specificity: The antibody is a rabbit polyclonal antibody raised against TRAF6. It has been selected for its ability to recognize TRAF6 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant TNF Receptor Associated Factor 6 (TRAF6) corresdonding to Ala153~Pro423 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TRAF6

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Alternative Name: TNF Receptor Associated Factor 6 (TRAF6 Products)

Background: RNF85, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway