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GCLC antibody (AA 435-636)

GCLC Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7435583
  • Target See all GCLC Antibodies
    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
    Binding Specificity
    • 9
    • 8
    • 7
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 435-636
    Reactivity
    • 60
    • 22
    • 19
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 59
    • 4
    Rabbit
    Clonality
    • 50
    • 13
    Polyclonal
    Conjugate
    • 37
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This GCLC antibody is un-conjugated
    Application
    • 45
    • 26
    • 21
    • 13
    • 11
    • 10
    • 8
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against GCLC. It has been selected for its ability to recognize GCLC in immunohistochemical staining and western blotting.
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) corresdonding to Asp435~Ser636 with N-terminal His Tag
    Isotype
    IgG
    Top Product
    Discover our top product GCLC Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:450-1800 Immunohistochemistry: 5-20 μg/mL

    1:45-180 Immunocytochemistry: 5-20 μg/mL

    1:45-180 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
    Alternative Name
    Glutamate Cysteine Ligase, Catalytic (GCLC Products)
    Background
    GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS
    Pathways
    Cell RedoxHomeostasis
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