MEK1 antibody (AA 54-369)

Catalog No. ABIN7435547

Product Details anti-MEK1 Antibody

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Binding Specificity: AA 54-369

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)

Specificity: The antibody is a rabbit polyclonal antibody raised against MAP2K1. It has been selected for its ability to recognize MAP2K1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) corresdonding to Leu54~Val369 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: Mitogen Activated Protein Kinase Kinase 1 (MAP2K1 Products)

Background: MAPKK1, MEK1, MKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling