CD3 epsilon antibody (AA 28-185)

Catalog No. ABIN7439360

Product Details anti-CD3 epsilon Antibody

Target: CD3 epsilon (CD3E)

Binding Specificity: AA 28-185

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD3e. It has been selected for its ability to recognize CD3e in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) corresdonding to Met28~Asn185 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-3 μg/mL

Immunohistochemistry: 5-30 μg/mL

Immunocytochemistry: 5-30 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: T-Cell Surface Glycoprotein CD3 Epsilon (CD3E Products)

Synonyms: cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody

Background: T3E, TCRE, CD3-E Molecule,Epsilon(CD3-TCR Complex), T-cell surface antigen T3/Leu-4 epsilon chain

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway