Sclerostin antibody (AA 25-213)

Catalog No. ABIN7435190

Product Details anti-Sclerostin Antibody

Target: Sclerostin (SOST)

Binding Specificity: AA 25-213

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sclerostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Sclerostin (SOST)

Specificity: The antibody is a rabbit polyclonal antibody raised against SOST. It has been selected for its ability to recognize SOST in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Sclerostin (SOST) corresdonding to Gly25~Tyr213 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:150-600 Immunohistochemistry: 5-20 μg/mL

1:15-60 Immunocytochemistry: 5-20 μg/mL

1:15-60 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-Sclerostin antibody (ABIN7435190)

Sun, Zhang, Chen, Ji, Ma, Qiao, Wu, Zhou, Bu, Zhu, Zhang, Jiang, Liu, Li, Liu, Yang, Liu: "Targeting SOST using a small-molecule compound retards breast cancer bone metastasis." in: Molecular cancer, Vol. 21, Issue 1, pp. 228, (2023) (PubMed).

Target Details for Sclerostin

Target: Sclerostin (SOST)

Alternative Name: Sclerostin (SOST Products)

Synonyms: LOC100313724 antibody, CDD antibody, VBCH antibody, 5430411E23Rik antibody, sclerostin antibody, LOC100313724 antibody, SOST antibody, Sost antibody

Background: VBCH, Sclerosteosis