PI3 antibody (AA 41-117)

Catalog No. ABIN7435181

Product Details anti-PI3 Antibody

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Binding Specificity: AA 41-117

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Peptidase Inhibitor 3, Skin Derived (PI3)

Specificity: The antibody is a rabbit polyclonal antibody raised against PI3. It has been selected for its ability to recognize PI3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Peptidase Inhibitor 3, Skin Derived (PI3) corresdonding to Phe41~Gln117 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Alternative Name: Peptidase Inhibitor 3, Skin Derived (PI3 Products)

Synonyms: ESI antibody, SKALP antibody, WAP3 antibody, WFDC14 antibody, cementoin antibody, trappin-2 antibody, TRAPPIN-2 antibody, elafin antibody, peptidase inhibitor 3 antibody, peptidase inhibitor 3, skin-derived (SKALP) antibody, PI3 antibody

Background: SKALP, ESI, WAP3, WFDC14, Elafin, Skin-Derived Antileukoproteinase, Cementoin, Elastase-specific inhibitor, WAP four-disulfide core domain protein 14