PDK1 antibody (AA 172-399)

Catalog No. ABIN7440905

Product Details anti-PDK1 Antibody

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Binding Specificity: AA 172-399

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDK1. It has been selected for its ability to recognize PDK1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) corresdonding to Val172~Pro399 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:250-1000 Immunohistochemistry: 5-20 μg/mL

1:25-100 Immunocytochemistry: 5-20 μg/mL

1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Alternative Name: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1 Products)

Synonyms: ATP5A antibody, B830012B01 antibody, D530020C15Rik antibody, si:zfos-979f1.3 antibody, 3-phosphoinositide dependent protein kinase-1 antibody, pyruvate dehydrogenase kinase 1 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9) antibody, pyruvate dehydrogenase kinase, isoenzyme 1 antibody, pyruvate dehydrogenase kinase, isozyme 1 antibody, pyruvate dehydrogenase kinase 1 L homeolog antibody, Pdpk1 antibody, PDK1 antibody, ATP5G2 antibody, Pdk1 antibody, pdk1 antibody, pdk1.L antibody

Background: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect